| Aspect | Details | |--------|---------| | | mimk58 resides on a 3.2 kb operon (designated mimK‑58 operon ) located on the megaplasmid pMIMK‑58 of Pseudomonas putida strain M58. | | Regulation | The operon is under the control of a σ⁵⁴‑dependent promoter activated by the transcriptional activator MimR in response to aliphatic hydrocarbon substrates. | | Evolutionary origin | Phylogenetic analysis indicates that mimk58 evolved via a gene‑fusion event that combined an ancestral Rieske‑type monooxygenase (≈30 kDa) with a C‑terminal leucine‑rich repeat (LRR) domain responsible for oligomerization. The fusion likely occurred in a marine sediment microbe exposed to petroleum hydrocarbons, providing a selective advantage for simultaneous degradation and bio‑film formation. | | Homologs | Distant homologs are found in Acinetobacter spp. and Rhodococcus spp., but none possess the exact 58 kDa size or the characteristic C‑terminal assembly motif. |
Addressing these issues will be decisive for translating laboratory successes into commercial technologies. mimk-58
The LRR domain endows MIMK‑58 with a : | Aspect | Details | |--------|---------| | |
In the world of professional audio, few identifiers carry as much weight as the number "58." Whether referring to the emerging CLAW Raag MI58 or the industry-standard Shure SM58 The fusion likely occurred in a marine sediment