Cells were seeded at 5 × 10³ cells well⁻¹ in 96‑well plates, treated with Mukd‑546 (0.1 nM–10 µM) for 72 h, and MTT (0.5 mg·mL⁻¹) added for 4 h. Absorbance at 570 nm measured cell viability. IC₅₀ values calculated using non‑linear regression (GraphPad Prism).
Mice were randomized (Day 7, when tumors reached ~100 mm³) to receive either vehicle (0.5 % methylcellulose) or Mukd‑546 (30 mg·kg⁻¹, oral gavage, QD) for 21 days. Tumor volumes measured twice weekly (V = ½ × length × width²). Body weight and clinical signs monitored. At endpoint, tumors were harvested for immunohistochemistry (IHC) of p‑ERK and Ki‑67. mukd-546
The film’s narrative centers on a "casual hookup" or "off-party" scenario where a group of men and women meet with the intent to explore uninhibited sexual desires. The plot emphasizes the transition from the tension of an initial meeting to a wild, collaborative environment. Key thematic elements include: Cells were seeded at 5 × 10³ cells
| Cell Line | Mutation | IC₅₀ (nM) | |-----------|----------|-----------| | MIA‑PaCa‑2 | KRAS‑G12C | 45 ± 3 | | PANC‑1 | KRAS‑G12D | 62 ± 5 | | A375 | BRAF‑V600E | 28 ± 2 | | SK‑MEL‑28 | BRAF‑V600E | 32 ± 3 | | A549 | KRAS‑G12S | 78 ± 6 | | HCT‑116 | KRAS‑G13D | 71 ± 5 | | Others (non‑mutated) | – | 210‑480 | Mice were randomized (Day 7, when tumors reached
Male Sprague‑Dawley rats (n = 4 per group) received Mukd‑546 either intravenously (2 mg·kg⁻¹) or orally (10 mg·kg⁻¹). Plasma samples collected up to 24 h, quantified by LC‑MS/MS (LLOQ = 1 ng·mL⁻¹). PK parameters (C_max, AUC₀‑∞, t₁/₂, bioavailability) calculated using Phoenix WinNonlin.